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Q5: There is still GST fusion protein eluted from the beads after chemical cross-linking, which interferes with pull-down experiment

They inject medication into specific muscle areas

The amount and distribution of this label reached its maximum after 30 min pulse length (Supplementary Fig

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Ferroptotic macrophages can intensify the inflammatory response by releasing pro-inflammatory cytokines and damage-associated molecular patterns (DAMPs)

If pain persists despite technique adjustments, the issue may be localized tissue sensitivity rather than injection mechanics